To confirm marker expression in live cells by immunocytochemical staining before colony selection.
Product Summary
Azide-free, stem cell marker antibodies conjugated to NorthernLights™ (NL) fluorochromes that can be used for single-step, direct immunocytochemical (ICC) staining of live, unfixed pluripotent stem cells.
Features
- Verifies marker expression before colony selection.
- Requires a short 30 minute incubation.
- Permits continued cell culture.
- Does not affect cell proliferation or stemness.
- Increases the probability of experimental success.
Species-specific Markers of Pluripotency
| Marker | Human Stem Cells | Mouse Stem Cells | Conjugated to NL493 (green) |
Conjugated to NL557 (red) |
| SSEA-1 | Negative Marker | Positive Marker | NLLC2155G | NLLC2155R |
| SSEA-4 | Positive Marker | Negative Marker | NLLC1435R | |
| TRA-1-60(R) | Positive Marker | Not applicable | NLLC4770G | NLLC4770R |
Data Examples
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Detection of SSEA-1 in Live D3 Mouse Embryonic Stem Cells. The stem cell marker SSEA-1 was visualized in live D3 mouse embryonic stem cells, grown on irradiated mouse embryonic fibroblasts (Catalog # PSC001), using a GloLIVE NL493-conjugated Anti-Human/Mouse SSEA-1 Monoclonal Antibody (Catalog # NLLC2155G; green). The cells were counterstained with Hoechst 33342 (blue). Positive staining for SSEA-1 in mouse stem cells is an indicator of pluripotency. |
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Detection of SSEA-4 in Live BG01V Human Embryonic Stem Cells. The stem cell marker SSEA-4 was visualized in live BG01V human embryonic stem cells using a GloLIVE NL557-conjugated Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # NLLC1435R; red). The cells were counterstained with Hoechst 33342 (blue). Positive staining for SSEA-4 in human stem cells is an indicator of pluripotency. |
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Detection of TRA-1-60(R) in Live BG01V Human Embryonic Stem Cells. The stem cell marker TRA-1-60(R) was visualized in live BG01V human embryonic stem cells using a GloLIVE NL493-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody (Catalog # NLLC4770G; green). The cells were counterstained with Hoechst 33342 (blue). Positive staining for TRA-1-60(R) in human stem cells is an indicator of pluripotency. |